Advances in Clinical Chemistry, Vol. 29 by Herbert E. Spiegel (ed.)

By Herbert E. Spiegel (ed.)

The sector of medical chemistry now encompasses features of analytical chemistry, biochemistry, medical laboratory technology, toxicology, immunology and molecular biology. This serial presents a extra entire international view of the present and destiny instructions of this increasing self-discipline. This quantity offers topical papers realted frequently to the kidney, melanoma and purposes of the polymerase chain response.

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Increase in the glucosylated form of erythrocyte Cu-Zn-superoxide dismutase in diabetes and close association of the nonenzymatic glucosylation with the enzyme activity. Biochim. Biophys. Acra 924, 292-296 (1987). A7. , Glycation and inactivation of human Cu-Zn-superoxide dismutas: Identification of the in vivo glycated sites. J. Biol. Chem. 262, 16969-16972 (1987). A8. , and Burr, I. , Joint purification of mangano and cuprozinc superoxide dismutases from a single source-A simplified method.

Anal. Biochem. 136, 336-339 (1984). A9. , Sharp, R. , and Burr, I. , Purification and radioimmunoassays for superoxide dismutases in the mouse: Tissue concentrations in different strains. Inr. J . Biochem. 17, 1171- 1178 (1985). A10. , and Burr, I. , Rat superoxide dismutases-Purification, labeling, immunoassay, and tissue concentration. J. Biol. Chem. 260, 2212-2217 (1985). A l l . , Induction of manganese superoxide dismutase by tumor necrosis factor in human breast cancer MCF-7 cell line and its TNF-resistant variant.

0.. M. 800- 7I i + I -I a 1 f 400- 2 f y z I- 200- z v) a 0.. 0, M. 17. Typical patterns of Mn-SOD release into serum for two male patients with acute myocardial infarction. (A) A case with an unsuccessful reperfusion; (B) successful case. The mean level for normal male adults is almost 100 ng/ml. Other enzymes: AST, ~-aspartate:2-oxoglutarate;ALT, ~-alanine:2-oxoglutarate; CK, creatine kinase; LDH, lactate dehydrogenase. 01 0 50 100 150 Time from the onset (hours) n 0 y 200. 18. Serial determinations of serum Mn-SODin 29 patients with acute myocardial infarction: (A) 23 cases with successful and (B) 6 cases with unsuccessful reperfusion.

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